Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

This protocol outlines the basic principles of PCR, provides a methodology that will result in amplification of most target sequences, and presents strategies for optimizing a reaction.

Protocols that do not use harsh and dangerous chemicals, e.g., phenol, are available online. They produce sufficient quality to enable most routine PCR applications.

View a protocol to perform PCR using Taq DNA Polymerase including materials, reaction setup, and thermocycling conditions for 25 µl and 50 µl reaction sizes.

Analyze the results of your PCR reaction via gel electrophoresis. GenScript tells you how to design PCR tools, and provides PCR schemes and PCR steps.

See our reference page on how to design primers for detailed instructions and check out our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes.

This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase.

1) 4.7 ul water 2) 1.25 ul 10x PCR buffer 3) 0.65 ul MgCl 2 4) 0.20 ul dNTP (25 mM each) 5) 1.0 ul primer 1 6) 1.0 ul primer 2 7) 0.2 ul Taq Polymerase Notes: · Use 1 ul template DNA or cDNA; …

The protocol in brief You will perform a PCR reaction on you DNA sample to generate multiple copies of a portion of the 16S rRNA gene. The first step involves setting up a master mix …

May 31, 2022 · The PCR process can be used for a wide variety of laboratory and clinical applications and purposes. Read about what happens at each of the process steps.