A hybrid bacteriophage, P420, was constructed in vitro; it contains part of bacteriophage P4 and a 3.6-kilobase derivative of plasmid ColE1. In Escherichia coli, the plasmid-phage hybrid can exist as ...

Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 15 (Jul. 22, 2003), pp. 8692-8697 (6 pages) The DnaA replication initiation protein has been shown to be ...

Toxin-antitoxin (TA) systems are now known to negatively control plasmid replication. Toxin-antitoxin (TA) systems are now known to negatively control plasmid replication, according to Thomas Wood, ...

Plasmids, small circular DNA molecules found in bacteria, may contain antibiotic-resistance genes and have the ability to replicate independently. Bacteria can transfer these plasmids to one another, ...

Plasmid DNA (pDNA) is a fundamental starting material for therapeutic development, including for viral vectors, mRNA therapies, and DNA vaccines. Researchers use capture methods such as anion exchange ...

DNA-based information is a new interdisciplinary field linking information technology and biotechnology. The field hopes to meet the enormous need for long-term data storage by using DNA as an ...

Adeno-associated virus (AAV) vectors play a pivotal role in gene therapy, delivering therapeutic genes with high precision and safety. However, a significant challenge in the biomanufacturing of these ...

Microbial systems offer distinct advantages in protein expression, saving costs and time, and offering well-characterized host genetics and ease of scale-up. 2 However, challenges remain in process ...

AGC Biologics has completed manufacturing improvements at its site in Heidelberg, Germany. With it comes the expansion of its plasmid DNA (pDNA) capacity. The Seattle-based CDMO says that it can now ...